Device

Part:BBa_K574006:Design

Designed by: Lei Wei   Group: iGEM11_Tsinghua-A   (2011-09-29)

3OC12HSL regulated by pTet and pBad


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 930
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 744
    Illegal BglII site found at 1625
    Illegal BamHI site found at 870
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 306
    Illegal AgeI site found at 1187
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We design this part to regulate the sending of the 3OC12HSL. L-arabinose acts as an inducer which we can add into the system and TetR acts as a repressor which comes from the system inside.

Source

We used BioBrick Standard Assembly. List as follows: BBa_R0040 BBa_K081016 BBa_I13453 BBa_K081009 BBa_E0430

References